PPT – Bacterial Transformation PowerPoint presentation | free to view - id: 14d701-NGYwZ. Conjugation (bacterial “sex”) involves the exchange of DNA through direct cell-to-cell contact or through a bridge-like connection between two cells called a sex pilus. preparation for the pglo lab. After transformation, the cells may express the acquired genetic information, which may serve as a source of genetic diversity and potentially provide … chapter 3. content. partnership for health and wholeness benjamin v. lozare, ph.d johns hopkins, BACTERIAL MENINGITIS - . Bacteria are used to copy DNA and make desired proteins . abe lab sequence. What if nothing grows on either LB plate? Transformation is a key process in molecular cloning, by which multiple copies of recombinant DNA molecules are produced. • Changing the genes and phenotype of a bacteria by uptake of foreign/new DNA • a natural process that bacteria have evolved in order to obtain DNA from their environment. • If so, did the gene of interest get inserted into the plasmid? Antibiotic Resistance • Resistant bacteria can survive in the presence of antibiotics • We will be using ampicillin • Our plasmid is resistant to ampicillin, Lac-Z gene • Lactose = glucose + galactose • Lactose is broken down by the Lac-Z gene • Lac-Z will also digest similar compounds, X gal • X gal is a compound that is similar to lactose • X gal turns blue when it is digested by the Lac-Z gene, Engineered Plasmids • We will be using pBlue Lac-Z gene Ampicillin Resistance gene, Restriction Enzymes • Our plasmid DNA and our foreign DNA arrive pre-cut by the same restriction enzyme • The restriction enzyme made a single cut in the plasmid • The restriction site is within the lac-Z gene, Restriction site Lac-Z gene Ampicillin Resistance gene, We will be using 3 types of agar • LB • LB + amp • LB + amp + X gal, Each Group Will Run Six Plates LB LB - plasmid + plasmid LB/amp LB/amp - plasmid + plasmid LB/am/X-gal LB/am/X-gal + plasmid - plasmid, LB Plates • There is nothing in the LB agar to interfere with growth. vaginitis curriculum. There are two versions of the PowerPoint: the (editable) original and a slideshow version (identical in content) suitable for upload to your VLE for student use. • To transform cells, you first need to make them competent to take up extracellular DNA. Only a fraction (less than 1 in 1000) will acquire the plasmid. Amp X-Gal pBlu pBlu Control Control Regular Amp X-Gal Regular, Growing the bacteria • After they have received the plasmid… • Place on a growth media and allowed to grow. Bacterial transformation is a key step in molecular cloning, the goal of which is to produce multiple copies of a recombinant DNA molecule. How do we get the plasmid inside of the bacteria? • We use a marker • Grow the bacteria on plates that contain the antibiotic • Use a color pigment marker that is present when a particular enzyme is present Let’s review bacterial DNA first…, Let’s Review: Bacterial genome • Bacteria are prokaryotes—no nucleus. • Wait…and then • Heat shock! jellyfish. Find PowerPoint Presentations and Slides using the power of XPowerPoint.com, find free presentations research about Microbial Transformation PPT What is Transformation?. introduction of plasmid dna into e. coli. Actions. In general, transformation is a complex, energy-requiring developmental process. It was first reported in Streptococcus pneumoniae by Griffith in 1928. Thus, cross contamination is common. If you continue browsing the site, you agree to the use of cookies on this website. Title: Bacterial Transformation Author: tanasj Last modified by: Berry, Paula Created Date: 1/23/2006 7:36:27 PM Document presentation format: On-screen Show (4:3) finger flick, Bacterial Transformation of pGLO Lab Background - . Slideshow 4651557 by mikaia change… in molecular biology, change, Bacterial Transformation Teacher Preparation Materials Lists - . 1. A circular piece of autonomously replicating DNA exists outside the main bacterial chromosome Originally evolved by bacteria Carries separate genes for specialized functions. A bacterial culture is the end result of bacterial multiplication in artificial media in the laboratory. what is our goal?. Natural transformation is often viewed as a way to acquire new genes and to generate genetic mixing within bacterial populations. recombinant transformation. Our Goals • Insert foreign gene into plasmid • Insert recombinant plasmid into bacteria • Grow transformed bacteria, Questions We Need Answered • Did the bacteria take up the plasmid? This is a lab report assignment to accompany the pGLO Bacterial Transformation Lab done in many AP Bio and Biotechnology classes. rna. Title: Ligation and Transformation Last modified by: 000762265 Created Date: 6/13/2005 1:05:43 PM Document presentation format: On-screen Show (4:3) – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 71e3f0-NzAxM Did the bacteria take up the plasmid? Bacterial Transformation Lab 6A. Essential Knowledge What must you know to understand this lab? How will we know if the bacteria actually got into the. • Should you ever see blue colonies on - ? Recombinant Transformation Open the tube of competent cells labeled “CC” and add 20 µl of the ligation solution “Lig” directly to the “CC” tube solution using a micropipettor and sterile tip. purification of cultures morphological and pureculture studies, Agrobacterium -mediated transformation of rice - . Applications of TransformationApplications of Transformation Bacterial transformation is used: •To make multiple copies of DNA, called DNA cloning. many organisms can fluoresce. Transformation is defined as the insertion of a gene into an organism in order to change the organism’s trait(s). To make bacterial cells competent for transformation, the cells are incubated in chilled calcium chloride. • If the Lac Z gene is interrupted, X-gal will not be digested and the colonies will remain white, insertedgeneof interest “broken”LacZ gene recombinantplasmid amp resistance If the foreign DNA is inserted into the plasmid, • The Lac-Z gene will be interrupted and bacteria will stay white LB + amp + X gal, Blue vs. White on X-gal Plate • Bacteria take up plasmid • Functional LacZ gene • X gal is being digested • Bacteria make blue color • Bacteria take up recombinant plasmid • Non-functional LacZ gene • X gal is NOT being digested • Bacteria stay white color, What if you just see blue colonies on + ? Bacterial transformation is an unavoidable step in . `acute infection of the cns the clinical syndrom : bacterial meningitis viral minigitis encephalitis brain, pGLO Bacterial Transformation - Dna. • Our plasmid contains genes for: • AMP= ampicillin (an antibiotic) resistance • Beta-galactosidase-an enzyme that converts X-Gal Indo Blu RNA Protein that allows for antibiotic resistance RNA Enzyme that breaks down X-Gal to make Indo Blu, How do we get the plasmid inside of the bacteria? Genetic Engineering. general transformation steps model, BACTERIAL MORPHOLOGY: PROCARYOTIC AND EUCARYOTIC CELLS Chapter 4 - . Why? green fluorescent protein. to express, Bacterial Transformation - . LB/amp/X-gal • Color of colonies on X-gal plate will tell you if gene of interest has been inserted into the plasmid or not • Blue colonies indicate Lac Z gene is digesting X-gal • White colonies indicate Lac Z gene in not functioning. Transformation in bacteria was first studied by a scientist Frederick Griffith in the year 1928. Which of the following results from Griffith's experiment is an example of transformation? • Roles of group members ... Microsoft PowerPoint - Bacterial Transformation Lab PPT opengl rendering pipeline. Obtaining a pure culture is essential in guaranteeing accurate and reliable laboratory experi-ments. Learn vocabulary, terms, and more with flashcards, games, and other study tools. genetic information, Bacterial Transformation with ( pGLO Plasmid) - . Competence refers to the state of being able to take up exogenous DNA from the environment. Bacterial Transformation. Bacterial Transformation. gebre k. tseggay, md november 21, 2005. major changes in epidemiology of meningitis since the, Bacterial Metabolism - . Our Goals. learn how to insert a, Bacterial Transformation - . transformation sentences 2. reformulate each sentence so that it includes the word in, CNS INFECTIONS - . 1 Bacterial Transformation 1 Biotechnology today. Get powerful tools for managing your contents. Oct. 28, 2020. Electrophoresis. an overview of metabolism. gene regulation, Laboratory: Bacterial Transformation - . bacterial transformation powerpoint, Bacterial transformation is a key step in molecular cloning, the goal of which is to produce multiple copies of a recombinant DNA molecule. • In order to transform bacteria we need to overcome two problems • Disadvantage –cells that contain plasmids grow more slowly • There is pressure on cells to get rid of their plasmids • Needs to be an Advantage to keep plasmids • Antibiotic resistance • How do we tell which cells have the plasmid? • Should you see isolated colonies or a lawn on the X gal plate? creating a recombinant plasmid. pglo plasmid. The Adobe Flash plugin is needed to view this content. • The area where DNA is located is called the nucleoid • DNA is organized in one double stranded circular molecule, Bacterial DNA Bacterial cell Plasmid DNA Genomic DNA. protein. lab #10: molecular biology. xiangbing meng 2009.04.02. outline. pglo bacterial transformation. cmsd's academic, Transformation sentences 1 - . Cloning. •Express the pGlo protein. by joyce simko, anita beebe, judy king and sr. clare marie klein. vertex shader. LB + amp + X gal SUCCESS! LB/amp Plates • What if your – shows unexpected growth? Times New Roman MS Pゴシック Arial Gill Sans Ultra Bold Wingdings Comic Sans MS Gill Sans UltraBold Arial Black Default Design 1_Default Design Bitmap Image DNA Technology: BACTERIAL TRANSFORMATION Gene Cloning PowerPoint Presentation PowerPoint Presentation What is Bacterial Transformation? rna. In genetic engineering, plasmids are one means used to introduce foreign genes into a bacterial cell. Prior steps for creating recombinant plasmids are described in traditional cloning basics and involve insertion of a DNA sequence of interest into a vector backbone. To make large amounts of specific human proteins, for example, human insulin, which can be used to treat people with Type I diabetes. •To make large amounts of specific human proteins, for example, human insulin, which can be used to treat people with Type I diabetes. Create stunning presentation online in just 3 steps. amphipods, pGLO Bacterial Transformation - Dna. wear gloves do, Bacterial Metabolism and Energy Generation - . Download Share What is carried on the Plasmid? finger flick, pGlo Bacterial Transformation Lab - . Chromosome Originally evolved by bacteria Carries separate genes for specialized functions wear gloves do, Biotechnology and Transformation! • cells lacking the ampR plasmid are sensitive to the use of on! 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